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nf-κb activation hitkit™  (Thermo Fisher)


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    Thermo Fisher nf-κb activation hitkit™
    Nf κb Activation Hitkit™, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nf-κb activation hitkit™/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    nf-κb activation hitkit™ - by Bioz Stars, 2026-05
    90/100 stars

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    MCMV inhibits TNF-α-induced transcription and NF-kB translocation in BM macrophages. (a) qRT-PCR analysis was performed to quantitate TNF-α-induced TRAF1 and ICAM-1 transcripts. The mean and standard error of the mean are shown from four independent experiments. (b) Confocal immunofluorescence studies were performed to monitor <t>TNF-α-induced</t> <t>NF-κB</t> nuclear localization in the presence or absence of MCMV infection. Results of one of three independent experiments are shown.
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    MCMV inhibits TNF-α-induced transcription and NF-kB translocation in BM macrophages. (a) qRT-PCR analysis was performed to quantitate TNF-α-induced TRAF1 and ICAM-1 transcripts. The mean and standard error of the mean are shown from four independent experiments. (b) Confocal immunofluorescence studies were performed to monitor TNF-α-induced NF-κB nuclear localization in the presence or absence of MCMV infection. Results of one of three independent experiments are shown.

    Journal:

    Article Title: Murine Cytomegalovirus Infection Inhibits Tumor Necrosis Factor Alpha Responses in Primary Macrophages

    doi: 10.1128/JVI.77.18.10125-10130.2003

    Figure Lengend Snippet: MCMV inhibits TNF-α-induced transcription and NF-kB translocation in BM macrophages. (a) qRT-PCR analysis was performed to quantitate TNF-α-induced TRAF1 and ICAM-1 transcripts. The mean and standard error of the mean are shown from four independent experiments. (b) Confocal immunofluorescence studies were performed to monitor TNF-α-induced NF-κB nuclear localization in the presence or absence of MCMV infection. Results of one of three independent experiments are shown.

    Article Snippet: BM macrophages were mock or MCMV infected for 18 h and then stimulated with TNF-α (10 ng/ml) for 30 min. Macrophages were then fixed, washed, and stained for NF-κB by using the NF-κB HitKit (Cellomics) per the manufacturer's directions, yielding green color (fluorescein isothiocyanate [FITC]).

    Techniques: Translocation Assay, Quantitative RT-PCR, Immunofluorescence, Infection